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Mouse Diets in Laboratories


The figures below are taken from diets fed to rats and mice in laboratory settings and have been calculated by scientists for the animals' nutrition. I cannot speak to the quality of the analysis since I am no scientist myself, but at least it may help give some guidelines about what sort of foods (and proportions) mice are regarded as requiring. Personally, I was surprised at the amount of wheat in the diet (knowing the problems that it can cause) and also a little at the alfalfa inclusion, since many mice will not eat this at all.

NIH Open Formula Rat and Mouse Diet 18% Crude Protein Autoclavable (NIH-31)

Ingredients
Percentage by weight
Fish meal (60% protein)
9.0
Soybean meal (48.5% protein)
5.0
Alfalfa meal (17% protein)
2.0
Corn gluten meal (60% protein)
2.0
Ground whole hard wheat
35.5
Ground #2 yellow shelled corn (8.5% protein)
21.0
Ground whole oats
10.0
Wheat middlings
10.0
Brewers dried yeast
1.0
Soy oil
1.5
Salt
0.5
Dicalcium phosphate
1.5
Ground limestone
0.5
Premixes
0.5
Total
100.00


Vitamin Fortification per ton (2,000 lbs) of finished product

Vitamin
Amount
Source
A
22,000,000 IU
Vitamin A palmitate or acetate
D3
3,800,000 IU
D activated animal sterol
K
20 g
Menadione
dl Alpha-tocopheryl acetate
15 g
Choline
700 g
Choline chloride
Folic acid
1 g
Niacin
40 g
d Pantothenic Acid
25 g
d-Calcium Pantothenate
Riboflavin supplement
5 g
Thiamin
65 g
Thiamin mononitrate
B12 supplement
40,000 mcg
Pyridoxine
5 g
Pyridoxine hydrochloride
Biotin
120 mg
d-Biotin

 

Mineral Fortification Per Ton (2,000 lbs) of Finished Product

Mineral
Amount
Source
Cobalt
400 mg
Cobalt carbonate
Copper
4 g
Copper sulfate
Iron
60 g
Iron sulfate
Magnesium
400 g
Magnesium oxide
Manganese
100 g
Manganese oxide
Zinc
10 g
Zinc oxide
Iodine
1500 mg
Calcium iodate

These concentrations of vitamins and minerals shall be added to the ration via two separate (vitamin and mineral) premixes. The final formulation may be adjusted so the total amount of ingredients will equal 100%. In the case of the mineral fortification, the actual amount of each element required is specified. Therefore, the contractor shall adjust the amount of each compound used in the premix according to its mineral concentration.

Nutrient Standards
Micro Analysis - The total calculated concentrations of nutrients in the ration from ingredients and from the fortifications at the time of manufacture should be as follows:

Crude protein
% Minimum
18.0
Crude fat
% Minimum
4.0
Crude fibre
% Maximum
5.0
Ash
% Maximum
8.0

 

Amino Acids (% of total diet)
Minimum
Arginine
.90
Lysine
.85
Methionine
.35
Cystine
.25
Tryptophan
.20
Glycine
.95
Histidine
.38
Leucine
1.40
Isoleucine
.95
Phenylalanine
.85
Tyrosine
.60
Threonine
.65
Valine
.90

 

Mineral
Minimum
Calcium
%
1.15
Phosphorous
%
.85
Potassium
%
.75
Sodium
%
.30
Magnesium
%
.15
Iron
PPM
345.00
Zinc
PPM
40.00
Manganese
PPM
140.00
Copper
PPM
12.00
Cobalt
PPM
0.70
Iodine
PPM
1.80


Vitamin
Minimum
Vitamin A
IU/g
20.0 (10)*
Vitamin D
IU/g
4.0
Alpha-tocopherol
PPM
45.0
Thiamin
PPM
70.0
Riboflavin
PPM
7.0
Niacin
PPM
80.0
Pantothenic acid
PPM
30.0
Choline
PPM
1900.0
Pyridoxine
PPM
10.0
Folic acid
PPM
2.0
Biotin
PPM
.2
Vitamin B12
mcg/Kg
40.0
Vitamin K
PPM
20.0

* TRUE VITAMIN A ACTIVITY BY HPLC METHOD

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